･The differential distribution pattern of Nτ-methylated proteins was found between the brain and skeletal muscle, and identified γ-enolase where the His-190 at the Nτ position is methylated in mouse brain.
･In silico structural prediction and biochemical analysis showed that the His-190 in γ-enolase is involved in the intermolecular homodimeric formation and enzymatic activity.
･γ-enolase was methylated in brain tissue extracts, indicating the presence of a histidine methyltransferase other than the three known ones in mouse brain tissue.
